IGEM:MIT/2005/Beginning of Week Meeting: Monday, July 5

Top Priority (can be done today)

1. Figuring out fusion point - where to attach ToxR to scFvs. Need help reading paper.
   • Why was this place chosen on other papers? Use this to help figure this.
   • What else did they try? What didn't work, why?
   • Look at references from article.
2. Specify this week -> next week's experiments.
3. Controls, what to expect
4. Getting cells from Harvard - learning about them, reading papers.
5. Order Biobricks
   • In parallel: order primers to make BioBricks in lab.
   • scFvs 1-3
   • ToxR

To Do This Week

• How to get library of fluorecein dimers
• Get ToxR and ToxR-cI plasmids from Harvard

Converting to BioBrickism

• Get rid of incorrect restriction sites, attach biobrick ends.
• See codon usage to ensure efficiency in transcription. (Take out rare codons)
• Biobrick domains. Allow for exchange of scFv easily.

Issues

• Protein domains - LD linker is fixed.