IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/10/10

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  • Minprep of all samples inoculated yesterday.
  • Gel run performed:
  • Blank x6; J+I7(1-1; 1-2; 2-1; 2-2); J+E2(1-1; 1-2; 2-1; 2-2)
  • Co transformations performed as follows:
  • J+I(1-1;1)(1C3) + J+E(1)(1A3)-------> 1
  • J+I(1-1;1)(1C3) + J+E(2)(1A3)-------> 2
  • J+I(2-2;2)(1C3) + J+E(1)(1A3)-------> 3
  • J+I(2-2;2)(1C3) + J+E(2)(1A3)-------> 4
  • J+E(1-1;2)(1C3) + J+I(1)(1A3)-------> 5
  • J+E(1-1;2)(1C3) + J+I(2)(1A3)-------> 6
  • J+E(1-2;2)(1C3) + J+I(1)(1A3)-------> 7
  • J+E(1-2;2)(1C3) + J+I(2)(1A3)-------> 8



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