IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/10/01

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Entry title Competent cells could not be prepared cause OD of cells shot up. inoculated J23119 + E0420 and J23119 + I13507. Inoculated DH5alpha for competent cell prparation. Transformation of: J23119 (amp and Chl) The controls C1 to C8 which did not work the other day due to contamination. J24674 from kit (plate 2; 9D); J24676 from kit (plate2; 5O) J23119 S/P digested and alkaline phosphatased.