IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/20

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  • Plasmid miniprep performed on the ligations which were done on 18/8. viz. the B set of composite parts.
  • Gel run was performed on these miniprepped DNA.
  • Not good results. Inserts were found to be bigger than the plasmid backbones
  • The following parts were sent for sequencing: (Primer: VF2 - 10pmole/microL; Template concentration - 100ng/microL)
  • 002 - A2;A5;A6
  • 003 - A6;A7
  • 004 - A3;A5
  • 005 - A4;A7
  • 006 - A1;A5
  • 001 - B1 to B6
  • 002 - B1 to B6
  • 003 - B1 to B4
  • 004 - B1 to B7
  • 005 - B1 to B7
  • 006 - B1 to B8




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