IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/20

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Plasmid miniprep performed on the ligations which were done on 18/8. viz. the B set of composite parts.
Gel run was performed on these miniprepped DNA.
Not good results. Inserts were found to be bigger than the plasmid backbones
The following parts were sent for sequencing: (Primer: VF2 - 10pmole/microL; Template concentration - 100ng/microL)
002 - A2;A5;A6
003 - A6;A7
004 - A3;A5
005 - A4;A7
006 - A1;A5
001 - B1 to B6
002 - B1 to B6
003 - B1 to B4
004 - B1 to B7
005 - B1 to B7
006 - B1 to B8