IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA
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PLASMID stands for Plasmid Locking Assembly for Sustaining Multiple Inserted DNA. This application introduces a new paradigm in gene regulation. The study is based on the concept of plasmid loss. Any episome introduced into the cell shows a segregational asymmetry accompanied with differential growth rates in the absence and presence of episome leading to an overall loss of the episomal unit in the absence of any maintaining selective pressure. It is hypothesized that by appropriately controlling the external selective pressures, we can control the direction of plasmid loss in the cell, modifying the existing gene regulation system in a pre determined manner. It is also hypothesized that introducing negative selective pressures against certain other directions of plasmid loss, in the form of constitutively repressed endotoxins will help streamline the regulatory system even further.
If successful, this study allows for exquisitely delicate and precise multifactorial control of gene regulation in the future. It is also expected that this model can be used to also hide genes of commercial interest to protect it from unauthorized use.