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Fast Response Module - Key issues - Priority
Quorum sensing mechanism
cross-talking pathways – heterodimerisation (heterodimerising pathways) in (G+)
Protease and fusion protein
Alternative split/ dimerizing proteases to use.
Alternatives: Phosphate-inducible/ allosteric activating proteases- look at the system, can we manipulate or use them some how?
Two Component system engineering
Synbio applications using EnvZ-OmpR 2CS (what hybrid receptors have been made to what stimuli) EnvZ receptor structure hybrid receptors that have been made and how they were assembled
animation for EnvZ- OmpR – TEV
Screen list of 2CS check for dimerization mechanism, how system is triggered, look for new ones (newcastle igem ref)
Search for gram positive 2CS, get a clear diagram and structural info of receptors if possible (Agr Com)
Contact structural biologist about OmpR-TEV protease fusion
Specificity of TEV protease and activity of split
Selection and engineer the link between the two – length of sequence – will it still FRET
List of biobrick parts
We could use- screen previous iGEM teams
2CS QS systems
Detection Module - Key issues
Induction of invasive behaviour
Specificity of schistosoma proteases
Could adding the protease recognition site to the AIP effect its properties enough to prevent it from binding the receptor?