IGEM:Imperial/2010/2010/07/20
Project name | Main project page Previous entry Next entry |
Fast Response Module - Key issues - PriorityQuorum sensing mechanismcross-talking pathways – heterodimerisation (heterodimerising pathways) in (G+) Protease and fusion proteinTEV protease
Alternative split/ dimerizing proteases to use. Alternatives: Phosphate-inducible/ allosteric activating proteases- look at the system, can we manipulate or use them some how?
Two Component system engineeringSynbio applications using EnvZ-OmpR 2CS (what hybrid receptors have been made to what stimuli) EnvZ receptor structure hybrid receptors that have been made and how they were assembled animation for EnvZ- OmpR – TEV
Screen list of 2CS check for dimerization mechanism, how system is triggered, look for new ones (newcastle igem ref) Search for gram positive 2CS, get a clear diagram and structural info of receptors if possible (Agr Com)
Contact structural biologist about OmpR-TEV protease fusion
Specificity of TEV protease and activity of split Fret pairSelection and engineer the link between the two – length of sequence – will it still FRET
Chassis
List of biobrick partsWe could use- screen previous iGEM teams 2CS QS systems Detection Module - Key issuesInduction of invasive behaviour
Specificity of schistosoma proteases
Could adding the protease recognition site to the AIP effect its properties enough to prevent it from binding the receptor? Membrane-anchored protein
|