IGEM:IMPERIAL/2009/Encapsulation/Parts/Nonleakypromoter/

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Target genes were cloned under control of the strong T7 promoter

T7 RNA polymerase production was placed under control of the E.coli lac promoter

Low levels of T7 lysozyme supplied by plasmids pLysS or pLysL [1]

image:T7lysozymeplasmids.jpg

The lower level of lysozyme provided by pLysS or pLysL has little effect on growth of the cell


Lysozyme binds to the T7 RNA polymerase present in the non-induced cell and prevents it from transcribing target genes, thereby avoiding the leaky expression of genes, which are under control of a T7 promoter

When the T7 RNA polymerase is induced,the production of RNA polymerase will exceed inhibition by lysozyme. Therefore, the T7 RNA polymerase will transcribe the target genes.

This has been used for expression of toxic malarial proteins in Escherichia coli [2]

Proposed application: Target genes= restriction enzymes

Useful references

Error fetching PMID 11606237:
  1. Error fetching PMID 11606237: [t7pol1]
Error fetching PMID 2023259:
  1. Error fetching PMID 2023259: [t7pol2]
Error fetching PMID 11080592:
  1. Error fetching PMID 11080592: [t7pol3]
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