IGEM:IMPERIAL/2009/Auto Encapsulating Pills
Diabetes is characterised by an inability to either respond to or produce sufficient insulin. We intend to remedy this by subcontracting pancreatic function to the intestinal epithelia.
1: Cultured Lactobacillus cells accumulate a protein product until a threshold level is reached.
2: Reaching the threshold is accompanied by deletion of the plasmid on which the protein is encoded.
3: Plasmid deletion is followed by expression of modified S layer protein which is encoded on the genome.
4: Cells are either separated based on charge differences or exposed to a super saturated solution and then separated based on encapsulation.
5: Encapsulation is intended for A: transport & storage B: Product dilution via division.
6: A certain amount of encapsulated Lactobacillus is administered orally.
7: Stomach acid removes capsule.
8: S layer facilitates intestine endothelial docking.
9: GLP 1 is secreted by docked cell.
Genes of Interest
Feedback from brain storming
Autoencapsulating cells Feed back: Look at how you define and make the genetic circuit more modular. Threshold detector/timer? Need inducible promoter or encounter problems. Whats the input output? How to evaluate performance? Timer: Customize delay… Thoughts: Big picture: Why could it be good to deliver protein based medication? MOTIVATION Getting hydrated proteins in. How much overexpression/surface coverage do you need to get a continous shell? KEY: SPEED AND UNIFORMITY. Self optimizing cells.
- Show a bit more about the genetic circuit (draw network) : Cell designer?/powerpoint?
- Encapsulation in yoghurt?
- Overall aim and big picture of the project?: Delivery of peptides,storage, motivation...
- New chassis? Possible challenges?