# IGEM:IMPERIAL/2008/Prototype/Drylab/Data Analysis/Track Analysis

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# Validation

A synthetic video of five cells with changing shape was used in the evaluation of tracking software. Each cell was tracked using respective tracking software, and their coordinates in pixels were obtained.

The following steps were taken to analyse tracking data:

1. Convert synthetic trajectory coordinate points into pixels.
2. Calculate the displacement in each frame for both synthetic and tracking data
3. Calculate the distance travelled in each frame for both synthetic and tracking data
4. Obtain the errors in coordinates, dislacement and distance travelled by the cell

The errors associated with manual tracking and Volocity software are shown below. All errors are given in terms of pixels.

## Manual Tracking

 Error in X Coordinate Error in Y Coordinate Mean = 1.7354, Standard Deviation = 1.7187 Mean = 1.2524, Standard Deviation = 1.0568 Error in X Displacement Error in Y Displacement Error in Distance Mean = -0.0022, Standard Deviation = 1.0002 Mean = -0.0018, Standard Deviation = 0.6795 Mean = -0.1506, Standard Deviation = 0.9196

## Volocity

 Error in X Coordinate Error in Y Coordinate Mean = -1.7149, Standard Deviation = 0.1376 Mean = -0.4907, Standard Deviation = 0.1614 Error in X Displacement Error in Y Displacement Error in Distance Mean = 8.2732e-005, Standard Deviation = 0.1111 Mean = 6.1416e-004, Standard Deviation = 0.0712 Mean = 1.4262e-004, Standard Deviation = 0.0947

# Evaluation

## Volocity

### Pros

• Lower error in terms of tracking centroid than other tracking software.
• Less time consuming.

### Cons

• Failure to track cells continuously over a long number of frames > 70
• Baised to cells which run, rather than tumble

## Manual Tracking

### Pros

• Able to discern cells visually
• Able to track over larger number of frames

### Cons

• Time consuming
• Higher error in terms of tracking centroid as compared to tracking software.

## Conclusion

• Manual tracking will be used to track B.Subtilis.