IGEM:IMPERIAL/2007/Projects/In-Veso/References

In-Veso Gene Expression: References

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

In Vitro Protein Synthesis

• In vitro protein synthesis (ambion)
• In vitro gene expression (promega)
• EasyXpress Protein Synthesis Mini Kit (Qiagen)
• Effects of growth rate on cell extract performance in cell-free protein synthesis
• In Vitro Synthesis of Protein in Microbial Systems

Cell Free Systems

1. Fletcher MP, Diggle SP, Cámara M, and Williams P. Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules. Nat Protoc. 2007;2(5):1254-62. DOI:10.1038/nprot.2007.158 |
2. Noireaux V, Bar-Ziv R, and Libchaber A. Principles of cell-free genetic circuit assembly. Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12672-7. DOI:10.1073/pnas.2135496100 |
3. Pellinen T, Huovinen T, and Karp M. A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter. Anal Biochem. 2004 Jul 1;330(1):52-7. DOI:10.1016/j.ab.2004.03.064 |
4. Noireaux V, Bar-Ziv R, Godefroy J, Salman H, and Libchaber A. Toward an artificial cell based on gene expression in vesicles. Phys Biol. 2005 Sep 15;2(3):P1-8. DOI:10.1088/1478-3975/2/3/P01 |
5. Noireaux V and Libchaber A. A vesicle bioreactor as a step toward an artificial cell assembly. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17669-74. DOI:10.1073/pnas.0408236101 |
6. Kim TW, Keum JW, Oh IS, Choi CY, Park CG, and Kim DM. Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system. J Biotechnol. 2006 Dec 1;126(4):554-61. DOI:10.1016/j.jbiotec.2006.05.014 |