IGEM:IMPERIAL/2007/Calendar/2007-9-13

Project Calendar

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Meeting with Prof Kitney

Team Logo

• Work on Sherlock Holmes 2007 theme (Silhouette with test tube?)
• Wiki main page design to have easy navigation icons
• Wet lab and dry lab pages to be linked back to CBD, ID and Home pages
• The rest of the wiki to retain professional layout for the scientific community

Vesicles

• Presented comparison between in-vitro and in-vivo
• Ties in with the bottom-up approach of synthetic biology
• So far achieved gene expression in vesicles (low yield)
• Try to relate vesicles to the CBD and ID applications
• Characterisation of CFS? (maybe focus on getting data for CBD and ID first)

Infector Detector

• DsRed from GeneArt is delayed because of quality control (scheduled to arrive early next week)
• Focus on infections caused by biofilms on urinary catheters (as opposed to other types of catheters)
• Final product detects biofilm by shining UV light on the catheters (try to simulate this in the lab?)
• Feedback to back up project with more experimental data in future presentations

Cell By Date

• Timeline of project depends on when DsRed arrives
  • takes ~1 week to get DNA construct, ~1 week for experiments, 1-2 weeks for data analysis and documentation
• Meanwhile continue experiments with GFP
• Changed specifications - 8°C, 2h (no need for 24-h experiments)
• Evaporation problem is minimised because of lower temperature and shorter time period

Next Meeting

• Next meeting with Prof Kitney and Prof Freemont to be scheduled for next week
• Aim to obtain some data for both CBD and ID by the next meeting