IGEM:IMPERIAL/2007/Calendar/2007-8-29

We are in Week 8 of the iGEM project

Project Calendar

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Debrief (Meeting with Professor Kitney)

Infector Detector

Comments

• This application is a big leap forward for the medical field.
  • If we perfect this application, we will be able to put it on every catheter.
• Think about the packaging of the system
• Does the in vitro system really give us the edge over the applications in the field of synthetic biology?
• How do we add LuxR (packaging)
• For the Jamboree presentation, take the audience through the research and application process step by step to make it easily digstible

In the lab

• Carry out this experiment in vivo and observe the edge it has over the in vitro application
• Is the DNA construc complicated enough?
• Define visible- DsRed Express and GFP; change the conditions ofobservation of the fluorescence produced
• How sensitive is the system?
• How long can the system bestored at e.g.4oC before usage?
• Problem with detection of low [AHL]
• How long can the system last? Can we optimize it?
• How stable is the fluorescent molecules?
• How robust is the system? e.g. temperature dependance, pressure dependant

Cell By Date

Comments

• Clarify experimental concept, and link it to meat spoilage
• Emphasize of DsRed Express as the reporter
• Think about the packaging of the product e.g. sticker form? in the cling film?
• Might have to look for another application other than meat spoilage , if construct does not fit the temperature range well

In the lab

• Place the device in a real scenario and observe how it works
• Find out the lag time of the system i.e. how long would DsRed Express take to reach visible threshold
• Construct does notfit the temperature range very well
  • Look into cryophilic bacteria, which has the appropriate cellular machinery to generate proteins at the temperature range of 0oC -5oC
• Look at improving the sensitivity (lag time) of the system
• Look into making new construct for pT7 as a biobrick from the current version we have so that we can swap bricks around later

Vesicles

Comments

• It is a new chassis
• If the pores used are toxic, should they be placed on the catheter?
• For the presentation, link this with the 2 applications
• Want to see the engineering cycle implemented into our project
• It is not essential to the main project, but serves as "icing on the cake"
• Less manpower should be devoted to it (reduce from 3 members)

In the lab

• It is very hard to calibrate fluoresence withinthe vesicle; if solution used is differet, vesicles would also behave differently
• It serves to increase the lifespan of the CBD application, but a strict control must be imposed on the quality

PR

• How do the 2 projects (applications) link together?
• Implement the engineerng cycle
• Sherlock Holmes theme is a good selling idea