IGEM:IMPERIAL/2007/Calendar/2007-8-23

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Debrief

Protocols

• Testing pLux in vivo tomorrow and also doing an initial test with the home made cell extract
• Clone tech purified sample of GFP is arriving wednesday and so calibration curves can be constructed on wed/thursday
• New results on our experimental design page
• Questions:Need to look for the pka of the GFP we are using as our cell extract is a pH of 8.2 and thsi could affect the system.

Vesicles

• New POPC lipids arrived.
• Continue to make and test vesicles
• Next step is to claify that the suspected vesicles formed are vesicles not mineral oil drops. Look into buying fluorescent lipids to test this

Lab

• Continue to make the reaction buffer for our home made cell extract tomorrow and should have it finished tomorrow.

Modeling Team

• Normalising the equation for infecter detector
• Next step to look at cell by date and initial data that is coming through from the lab.