IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation

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Super Parts Predator Construct
Actual Part
Sub Parts <bbpart>R0062</bbpart> <bbpart>B0034</bbpart> <bbpart>C0062</bbpart> <bbpart>I13504</bbpart>


Ligation strategy

Ligating the Polycystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37016</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)
Image:J37016 logo.png

Ligating the Monocystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
Image:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
Image:R0062 logo.pngImage:B0034 logo.pngImage:C0062 logo.png
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
Image:J37031 logo.png
<bbpart>J37032</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)
Image:R0062 logo.pngImage:I13504 logo.png
<bbpart>J37020</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)
Image:J37020 logo.png
  • More information on ligations can be found on the Ligation Page.

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37016</bbpart>
Image:J37016 logo.png Yes Yes Primers
<bbpart>J37019</bbpart>
Image:J37019 part.png Yes Yes Primers
<bbpart>J37020</bbpart>
Image:J37020 logo.png No N/A N/A
<bbpart>J37031</bbpart>
Image:J37031 logo.png Yes Yes Primers
<bbpart>J37032</bbpart>
Image:J37032 logo.png Yes No N/A
<bbpart>J37033</bbpart>
Image:J37033 logo.png Yes No N/A

WetLab work entries

  • The polycystronic predator sensing part (J37016) has been successfully built, and tested.
  • The monocystronic predator sensing part (J37020) was not built.
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