IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Prey Construct/Design

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Super Parts Prey Construct
Actual Part
Sub Parts F2620 C0261 I13401


Registry

J37015

Image:J37015.JPG

J37015 @ MIT Parts Registry

Design Choices

  • Design allows for an exponential increase of AHL via a positive feedback loop
    • Interaction between LuxR, AHL and promoter controlling expression of LuxI
  • Design allows for assesment of status of positive feedback loop via:
    • GFP Production - Linked to LuxI expression
    • AHL Production - Can be indirectly assessed via T9002 assay


INPUTS biological component comments
AHLLuxR + pLuxRThese components sense input and activate output
OUTPUTS biological component comments
AHLLuxILuxI expression causes enzymatic generation of AHL
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