IGEM:IMPERIAL/2006/LabCalendar/2006-9-3

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TO DO:
*Testing of T9002, J37016, J37020, S01656, J37015, J37015RS
*Mini & maxiprep of J37022 and J37025 (CHECK whether ligations were successful)
*Ligation for the final predator: J37019 + J37025  & subsequent electroporation
*Repeat ligation of J37018 ? (has not been completed & successful yet !)

Contents

Maxipreps

  • Maxipreped J37022(1) (colonies a&b), J37025(3) (colonies b&c), J27025(4) (colonies b&c).
  • Unable to do all 11 maxis due to lack of maxiprep supplies.

Minipreps

  • Minipreped J37022(1) (colonies a,b,c,d), J37025(3) (colonies a,b,c,d), J27025(4) (colonies a,b,c,d)

Ligations

  • Re-ligating J37018
  • Ligating J37036 (one of the final ones...)

Culturing

  • Cultured up 2ml of T9002, J37015, J37015RS, J37016 and J37020 from frozen stick
  • Cultured up 100ml of aiiA from Dr.Frey in LB medium for maxiprep
  • Cultured up 2ml of 4 different J37022 colonies
  • Cultured up 2ml of 4 different J37025 colonies
  • Cultured up 2ml of S01656 + AHL + IPTG
  • Cultured up 2ml of S01656 + AHL - IPTG
  • Cultured up 2ml of 1M as a control + AHL
  • Cultured up 3 colonies of S01656 in Rosetta cells in 5ml of LB
  • Cultured up 3 colonies of S01656 in BL21 cells in 5ml of LB

Electroporation

  • Had a bit of problem with continual blowing up, so used Kirsten's machine
  • Successfully electroporated J37031 + 9G
  • Unsuccessful for J37025 + J37019 (repeat tomorrow morning
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