IGEM:IMPERIAL/2006/LabCalendar/2006-8-13
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To do today
- Miniprep (37015RS & J37016)
- Reculture and miniprep cre 1, 2, 4 & AiiA
- Culture T9002 on request by Tom (2 mL M9, 2 mL LB)
Re-miniprep (J37015RS & J37016)
- L2, 3, 4
- M1, 2
- V1, 2, 3
- R1, 2
- Miniprep product in fridge to be digested tomorrow (pending acquisition of PstI and more EcoR1)
- 1 Day delay expected
Cultures
- Culture in Liquid Media
- Cre 1, 2, 4 (2 colonies each)
- AiiA (4 colonies)
- 12D->2H (4 colonies)
End of Sunday
- Cultured Cre 1, 2, 4, AiiA and 12->2H in 100ml for maxiprep tomorrow
- Cultured T9002 in 2ml of LB and M9 for testing tomorrow
- Failed to miniprep Cre 1, 2, 4, AiiA and 12->2H
To do tomorrow
- Complete digestion of J37015RS, J37016, Cre 1, 2, 4, AiiA, 12D->2H and run gel (from miniprep)
- Maxiprep Cre 1, 2, 4, AiiA, 12D->2H in parallel with
- T9002 testing