IGEM:IMPERIAL/2006/LabCalendar/2006-7-26

From OpenWetWare

Jump to: navigation, search


For Maxiprep

  • F2620 well 6B
  • C0261 well 24A
  • I13504 well 12D

Transferred to 2mL LB liquid culture in preparation for Maxiprep - in 37C shaker

For Miniprep

  • I13033 well 2H
  • J04500 well 16P
  • C0060 well 5M

Above transfereed to 2mL LB liquid culture in preparation for miniprep - in 37C shaker

  • R0062 well 9G
  • B0034 well 3O
  • C0062 well 7A

Above transferred to 2 mL LB liquid culture in preparation for miniprep. Separately, from previous miniprep, took 10 uL out to digest and to run gels later..

  • Made up 1% agarose solution, 60 mL ready for 3 gel plates, 2 mL ethidium bromide, and set to cool
  • Added TAE to prevent from drying out
  • A/O 12pm, the three digests are in 37C water bath before moving to gels in an hour

Prepare the following parts for Maxiprep and ligations for tomorrow

  • I13033 well 2H
  • C0062 well 7A
  • F2620 well 6B
  • C0261 well 24A
  • R0062 well 9G
  • B0034 well 3O
  • I13504 well 12D

Culture 100 mL LB and 200 uL bacteria with part in 500 mL flask. Set overnight in 37C shaker to culture.

Personal tools