IGEM:Harvard/2007/Meetings/Week 9
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Lunchtime meeting, 8/17
Harvard 2007 iGEM Jamboree Presentation Planning Meeting 1, 8/17
Content
Introduction
- How cells physically interact with environment?
- Underutilized aspects of iGEM: random libraries, surface expression
- Figure of overall project: "Harvard iGEM 2007"; overall fusion of project
Targeting
- Random library
- Loop/terminal
- Targets
- Nickel
- Calmodulin
- EGF
- GST
- His
- Strep
- PDZ
- Figures: Plates: before and after enrichment, somehow present numbers (pie chart? bar graph?), microscopy images with beads and fluorescent cells accumulating
Cell-cell signaling
- QS – sender, receiver = biobricks, back to initial idea: target + QS, with figures
- Figures: drop experiment (halo)
Intracellular signaling
- Advantage of Fec system = gene expression with low level of background – need specificity with targets, etc, regulation on cell level rather than on population level
- Figures: 3d structure, where and why we chose to alter loop region
Conclusion
- “Big goals” – medical applications
- Self-regulated production of certain proteins – “preprogrammed assembly”
- Constructs / futures
SLIDE allotment
- allow 45 seconds or so per slide
- total of 15 ish slides
- Intro = 2 slides
- Slide 1: Why do we want to do this?
- Slide 2: This is how we're going to do it; architecture of project
- Targeting = 4
- Cell-cell (QS) = 4
- Intracellular signaling = 3
- Conclusion = 2
TIME allotment
- Intro: 1 min
- Targeting: 3 min
- Cell-cell signaling: 2.5 min
- Intracellular signaling: 2.5 min
- Conclusion: 1 min