IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-27

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Possible PEG precipitation titration

(if Val's doesn't come out, or if would like more precision...)

trial folding reaction (μL) 20% peg / 2.5 M NaCl (μL) water (μL) final PEG concentration
110464%
210555%
310646%
410737%
510828%
610919%
71010010%

Gel analysis

2% agarose
2% agarose
lane contents
11kb+ ladder
210 μL nanostructures
3trial 4 pellet
4trial 4 supernatant
5trial 5 supernatant
6trial 5 pellet
7trial 6 supernatant
8trial 6 pellet
9trial 7 supernatant
10trial 7 pellet
11trial 8 supernatant
12trial 8 pellet
13trial 9 supernatant
14trial 9 pellet
15trial 10 pellet

results

  • it's difficult to draw any firm conclusions because of the poor quality of the gel, but it appears that increased concentrations of PEG lead to increased precipitation
  • where next: higher concentrations of PEG?
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