IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-12-19

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Western blot w/8% TrisGly

  1. 1: left corner cutout, right square cutout. Interaction
 1:L
 2:C(OD.95)
 3:BC(OD.95)
 4:AC(OD.95)
 5:ABC(OD.95)
 6:C(OD.95)  
 7:BC(OD.95) 
 8:AC(OD.95)
 9:ABC(OD.95)
 10:L
  1. 2: Oscillation
 1: sample 0 (t=0)
 2: sample 2 (t=60)
 3: sample 4 (t=120) etc..
 12: sample 22

Prepared more 1:5000 antiC in 50%Odyseey/50%PBST and 1:5000 anti-chicken.

2h antiC + ~5h antiC@4C.

Post antiC wash 3 x 10m 50%Odyseey/50%PBST Post secondary antichicken IR700 wash in 1x10minPBST 1x10minPBST 1x5min PBS Water rinse

Image:Cyano notebook.xls

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