IGEM:Cambridge/2008/Notebook/Voltage/2008/07/29

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  • Mutant E.coli strains had grown on plates.
  • Transferred mutants to liquid culture.
  • Primers arrived! Prepped as described in Protocols page
  • PCR started with OsmY (from Biobrick plasmids) and KDP (from E. Coli MG1655 Cell Culture)
  • E-Gel (SYBR) to confirm OsmY
  • E-Gel (EtBr) to confirm Kdp and OsmY
Gel showing KDP (2 thick bands on the left and OsmY (dimmer bands to the right)
Gel showing KDP (2 thick bands on the left and OsmY (dimmer bands to the right)



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