IGEM:Cambridge/2008/Notebook/Voltage/2008/07/24

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Double Terminator

The double terminator was made from BBa_B1006 and BBa_B1002, combined together.

First the two Biobricks were cut using the fast digest enzymes, at either Xba or Spe. They were then run on an agarose gel, cut out and extracted, using Zymoclean gel extraction. The 2006 was not cut succesfully with Spe, so was not extracted. The samples were then ligated, using fast ligation, such that 1002-1002 and 1002-1006 were hopefully produced. This was done by combining the 1002 cut with Spe with the 1002 cut with Xba and the 1006 cut with Xba. These were then run on an agarose gel and cut out, then frozen overnight.


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