IGEM:Cambridge/2008/Notebook/Voltage/2008/07/23

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Wet Work

  • Recovered Biobrick plasmid YFP from previous culture.
    • 1.6 ml of cells according to Zippy Plasmid Miniprep Kit protocol (60μL Elution Buffer used)
  • Added 20ml LB to 10ml innoculation of E Coli MG1655 (see 22/07/08) to increase volume in preparation for experiments tomorrow on K+ tolerance in media.




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