ROS Bursts
- Test catalase deficient strains
- Test other oxidases
Integrated Prophage
- Design/order lamB primers
- Find spo2 sticky ends
- Pick up a wt subtilis strain (Elowitz Lab) and find a good promoter
- Choose a selectable lysogenic phage
- Coli→subtilis conjugation?
- Constitutive recA
- Figure out transformation protocol for 50kb plasmid
Population Variation
- Primers to amplify promoter with different 5' lengths
- fimE plasmid (Keasling/Arkin labs)
- Find fimE binding sequence
- Order binding site oligos (new BioBrick parts)
- Natural SSM examples
Vitamins
- Primers to amplify lactis folate enzymes
- Do lactis RBS's work in coli?
Lactose Intolerance
- Primers to amplify and mutate lacY
- lacY+ strain (for a lacY template)
- Get, then BioBrick holin/lysozyme genes
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