IGEM:British Columbia/Protocols
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British Columbia iGEM Team Protocols
Below is a list of the common protocols that we have used during the year:
- Making Plates or Liquid Media
- Making Glycerol Stocks
- Transformations - Adapted from | Openwetware
- Day/Overnight Cultures
- Amplifying Biobrick Parts - Modified from Registry Instructions
- Restriction Digest - From the Endy Lab
- Biobrick Ligations - A combination of the Endy Lab protocol, OOW Consensus protocol and personal experience
- Colony PCR
- Gel Verification
- 3A Assembly - Modified from OWW Protocol
- Miniprep
- Competent Cell Preparation
