IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/30

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Results of Competent Cells Testing The DH5α A and B stocks and DB3.1 cells all had red colonies that were too numerous to count All cells are competent, the few cell count on previous testing was likely due to the pUC19 plasmid Glycerol stocks of A and B stocks DH5α cells were made by: Adding 0.618mL 100% glycerol to 3.8mL competent cells to make a 14% glycerol solution Store in -80°C freezer Transformation of pBADwt+RFP and pBADweak+RFP into BW27783 As pBADwt+RFP and pBADweak+RFP are currently just in DH5α glycerol stocks, need to transfer them to BW27783 cells which will have homogenous induction in the population upon stimulation with arabinose The plasmids were transformed using the iGEM:British Columbia Transfromation protocol with 3μL of miniprepped pBADwt+RFP and pBADweak+RFP A control transformation performed under the same conditions except for 1μL of 5ng/μL J23100 miniprepped plasmid was used J23100 cells were plated on Amp pBADwt+RFP were plated on Chlor pBADweak+RFP was plated on Amp-Tet