IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/29

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Testing Competent Cells Competent cell stocks A and B and a DB3.1 aliquot were transformed with J23100+RBS using the iGEM:British Columbia transformation protocol to determine if the cells are competent Testing pBAD weak+RFP and pBAD wt + RFP To test if the mutation of pBAD will work, both strains are grown in three liquid cultures containing LB+antibiotic and 0.01% arabinose, 0.1% arabinose and 1% arabinose After 3 hours, no red was seen in the pBAD weak+RFP strain. All three cultures of pBADwt started to express a similar level of RFP that could be seen with the human eye. After 5.5hours all three cultures of pBADwt showed a stronger color of red. The 1% and 0.1% arabinose cultures of pBAD weak expressed some RFP but at lower levels then pBADwt, 0.01% arabinose culture did not show any red that could be seen After 12 hours pBADwt was not significantly darker in color. pBAD weak exprssed more RFP, but was still considerably lighter then the pBAdwt strain NOTE: pBADwt+RFP was in BW27783 cells and pBADweak+RFP was in DH5a cells, this may have contributed to the color variation.