IGEM:American University/2009/Notebook/Advanced Experimental Chemistry: Fun Times in Beeghly/2013/04/03
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Welcome to Advanced Experimental ChemistryToday's ProcedureReaction the Hydrogels with Fluorescent Dye:
Dye Calculatations
Mass of cut hydrogel
Copper sulfat concentration
L-Ascorbic acid
range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso
To Do for Next TimeThursday: Take the films out of the dye. Put them in water overnight. Friday: Take fluorescence of the water bath to see if any dye leeched out (hopefully none did). Crosslink QAS films. Monday: Start growing Bacterial cultures. DSC old hydrogels and crosslinked films. Make more hydrogels (w butyn-1-ol) Tuesday: Start growing bacteria on the QAS films.
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