Haynes Lab:Notebook/TB Biosensor/2012/06/28
Project name | Main project page Previous entry Next entry |
Entry titledigested LuxS and LsrA minipreps from 6.27.12, crtE,I,B miniprep from 6.21.12 and Dr. Haynes' V0120 plasmid with Xba1 and Pst1 ran on a gel, cut out, extracted made chloramphenicol stocks in ethanol at 20mg/ml. Ernesto and I poured Cmp and Kan LB plates.
I told Ernesto to cut the wrong bands (I think he cut out the backbone!) I picked more colonies from RBS+LuxS and LsrA, put into 2ml of LB+amp, shook overnight
|