Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Genes/2012/06/17

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Gene Coordinates/Additional Cell Lines

  • These will be the gene coordinates (+/-250bps of the promoter region) to be used every time a calibration for a cell line is needed
  • Note: These coordinates are based off of the RefSeq genes

Image:Screen_Shot_2012-06-17_at_8.47.49_PM.png

  • Behzad's U2OS cell line, which is cancerous, is having problems picking up any signal for commonly known silenced genes.
  • I ran a calibration on other cancerous cell lines, and I seem to be having the same problem. Refer to paper: http://cancerres.aacrjournals.org/content/67/11/5097.full

Image:Screen_Shot_2012-06-17_at_8.53.25_PM.png



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