Haynes Lab:Notebook/Shawn Cloning bootcamp/2015/02/19

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Entry title DNA Concentration Data Plasmid OD260 OD260/280 ng/μL 1. Plasmid 1 .4355 1.901 435.2575 2. Plasmid 2 .186 1.9245 186.0375 OD260/280 measures the purity if the sample which should be around 2. ng/μL measures how much product was yielded. Restriction Digest Table Checked plasmid minipreps with EcoRI/PstI digests Reagent Volume Expected: 1. Plasmid 1 = 1500 2. Plasmid 2 = 4500 15 μL/lane; 1% agarose; Ladder DNA(plasmid) 3.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 8.5   15 μL --> 37°C/ 15 min. DNA Sequencing Samples Submitted to DNASU on: 'date' Order Number: Plasmid 1 - forward primer 'name' Plasmid 1 - reverse primer 'name' Plasmid 2 - forward primer 'name' Plasmid 2 - reverse primer 'name'
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