Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2016/08/22

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Overview

Transfected K562 cells on 8/20, following same protocol as before (from 2015-06-03).

K562 KAH132 Results - Microscopy

24hr

Well 1:


Well 2:


Well 4:


Well 5:



48 hr

Well 2:


Well 5:


K562 KAH132 Results - Flow Cytometry

24 hour:
Image:Screen_Shot_2016-09-20_at_11.10.45_AM.png


48 hour:
Image:Screen_Shot_2016-09-20_at_11.10.50_AM.png


Sample ID % RFP
24hr A84.8
24hr B84.9
48hr A28
48hr B26

K562 RNeasy prep

Harvested cells today, RNeasy prep. Concentrations/calculations for cDNA synthesis:

Sample ID ng/µL µL for 2 µg RNA (8 µL max) µL H2O qPCR Sample ID
K562 KAH132 24hr 19172.25.8K562_E008
K562 KAH132 24hr 28372.45.6K562_E009
K562 KAH132 48hr 18792.35.7K562_E010
K562 KAH132 48hr 26383.14.9K562_E011

Following steps from cDNA synthesis protocol.



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