Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/18

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K562 transfection results Main project page
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Overview

Here's the images taken 3 days after transfection of K562 cells.

KAH187-MV8 (positive control):

KAH126-MV2:

KAH87-MV10:

Almost no transfection of the test plasmids, and low transfection of the positive control. Could be due to high passage number (was on PS-9). Or cell density is less than optimal. Need to design an optimization experiment.



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