Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/03/11

From OpenWetWare

Jump to: navigation, search
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Main project page
Previous entry      Next entry

Summary

In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.



Goals

  • ✓ measure K562 concentration using flow cytometry
  • ✓ transfect K562 with KAH126-MV2 plasmid using lipofectamine 3000
    • ✓ take care to use correct cell density, and do not disturb the cells after adding lipofectamine mixture.



Protocol

example protocol




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

K562 culture concentration: 516,000 cells/mL

To get to 500,000 cells total per well, dilute 4-fold in fresh medium. 6mL culture, 18mL medium.



Personal tools