Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/16

From OpenWetWare

Jump to: navigation, search
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Main project page
Previous entry      Next entry

Summary

In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.



Goals

  • ✓ Measure K562 concentration using flow cytometry
  • ✓ Passage K562
  • ✓ Split K562 for nucleofection tomorrow, diluted to between 1E5 and 2E5 cells/mL



Protocol

flow cytometry

passaging/splitting cells




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Cell density of K562 culture measured at 9.5E5 cells/mL. To get under the 2E5 cells/mL target, will dilute 5-fold for the split being used for nucleofection. Will dilute the regular passage 10-fold.



Personal tools