Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/06

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RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Main project page
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Summary

Taking care of cell culture maintenance today, nothing special.

Goals

  • Passage SKNSH cells from PS-2 to PS-3
  • Split SKNSH cells into another 6-well plate, if available
  • Begin DH5α transformation for plasmid miniprep



Protocol

passaging adherent cells

transformation of E. coli with plasmid DNA (quick method)



Materials

  • List materials here, to be gathered before the experiment
  • Consumables such as media, antibiotics, DNA, kits etc.



Notes

Observations, measurements, placeholder info etc. go here



Results/Conclusions

Passaged SKNSH from PS-2 to PS-3. Some cells starting to show signs of changing morphology, clustering.

DH5α plates (one plasmid, one negative control) are in the bacterial incubator in the main lab.

To do: Transfect SKNSH cells in 6 well plate tomorrow, continue plasmid prep.



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