Haynes Lab:Notebook/Jan/2015/04/01

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Fluorescence Microscopy of Transfected U2OS Cells w/ BD006 Cell Line: U2OS Plasmid name: BD006 in MV2 Benchling Link for Plasmid: BD006/Chromatin Sensor 1 Name of Kit Used for Transfection: Lipofectamine LTX FACS needs to be performed to get exact transfection efficiency, but based on pictures, suspected efficiency is 20-30%. Cell morphology does not look great, probably due to the fact that there were few cells on the 6-well plate at the start of transfection.