Haynes Lab:Notebook/Jan/2014/09/18

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Extraction of KAH126 Name: KAH126 Date of culture inoculation: 9/17/14 Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success. Results for Streaks 1 and 2: Growth in both areas along zig-zag line, although the growth is splashed across the dividing line. Overall success. Sample 1: KAH126 colony 1; Sample 2: KAH126 colony 2; Sample 3: KAH126 (control DNA); DNA Concentration Data Plasmid OD260 OD260/280 ng/μL 1. Plasmid 1 2. Plasmid 2 Restriction Digest Table Checked plasmid minipreps with EcoRI/PstI digests Reagent Volume Expected: 1. 2. 15 μL/lane; 1% agarose; Ladder DNA(plasmid) 3.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 8.5   15 μL --> 37°C/ 15 min. DNA Sequencing Samples Submitted to DNASU on: Order Number: