Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/02/03

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Overview

Submitting samples for sequencing.

Sample ID Conc. (ng/µL) Amt for 150 ng (µL) Primer Primer amt (2 µL) H2O (µL) Sequencing # Sequencing ID
EED_v0120 11231.2P000122.811_EED_v0120 1_P0001
EED_v0120 11231.2P000222.822_EED_v0120 1_P0002
EED_v0120 3991.5P000122.533_EED_v0120 3_P0001
EED_v0120 3991.5P000222.544_EED_v0120 3_P0002
EM7:ZeoR_v0120 11201.3P000122.855_EM7:ZeoR_v0120 1_P0001
EM7:ZeoR_v0120 2612.5P000121.566_EM7:ZeoR_v0120 2_P0001
EM7:ZeoR_v0120 31341.1P000122.977_EM7:ZeoR_v0120 3_P0001
MV8 23620.4CMV fwd23.688_MV8 2_CMV fwd
MV8 33930.4CMV fwd23.699_MV8 3_CMV fwd
MV8 43230.5CMV fwd23.51010_MV8 4_CMV fwd



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