Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/11/04
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Phusion PCR on LCR fragment.
Reaction volume: 100 µL (split into 4 tubes to be pooled later)
All primers have annealing temperature at 60°C
Thermal cycler program:
Nonspecific binding and amplification in the sample. Not sure what's going wrong here... this is after PCR of the LCR product, using forward primer for HA1 and reverse primer for HA2. Product size should be approximately 2600-2700bp