Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/11/02

From OpenWetWare

Jump to: navigation, search
Today's project is... Main project page
Previous entry      Next entry

Overview

PCR results: DNA quantification and gels.

DNA Quantification

Sample ID 260/280 ng/┬ÁL
HA1 (PCR cleanup)1.839149.34
HA2 (PCR cleanup)1.868127.448
EM7-ZeoR (PCR cleanup)1.875234.784
HPK-CFP (PCR cleanup)1.877236.064
HPK-CFP (gel extraction)1.88724.101

Gel Image

From left to right: 1kb+ ladder, HPK-CFP, HA1, HA2, EM7-ZeoR

Cut out the large band from HPK-CFP and performed gel extraction, see above.



Personal tools