Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/06/08

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Gal4EED-luc HEK293 culture thawing Main project page
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Overview

Results of sequencing of DBN007 are back, sequence results match expected sequence.

Started a PS-α culture of Gal4EED-luc HEK293 by thawing cells in DMEM with 20% FBS, 1% p/s. Will switch over to a 10% FBS, 1% p/s and 1% puromycin medium when expanding into PS-β.


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