Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/05/06
|PCR of DBN006, adding IIS sites|| Main project page|
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Today I'll be performing PCR on the DBN006 plasmids I validated yesterday (from colonies 1 and 3), adding IIS restriction enzyme cut sites on their ends so they can be inserted into the DBN001 fragment containing homology arms.
Once PCR is complete, take 27 µL of each product, add 3 µL of 10x loading dye and verify product size via gel electrophoresis. If band matches, proceed with gel purification.