Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/01
|DBN001_pSB1A3 Cloning Results|| Main project page|
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Here are the transformation results from last week's digestion and ligation of DBN001 (gBlock) insert into the pSB1A3 vector, replacing GFP:
Could be a wrong choice of plasmid, or something went wrong during the digestion/ligation. Will repeat the process after doing a test digest on the plasmid to make sure the cut sites are there. Should see about using replacement plasmid, maybe one with RFP instead of GFP, as that's easier to visualize.
DBN002 f2 and f3 primers have arrived, as well as gBlock for and rev primers. To do tomorrow: