Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/07/28

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Gel Electrophoresis

  • 1% Agrose gel
    • 6μL sybr gel stain
  • Digests
    • DNA - 5.0μL
    • 10x FD buffer - 1.5μL
    • ECO RI - 1.0μL
    • Pst I - 1.0μL
    • dH2O - 6.5μL
  • Sample 1: DNA from colony 1
  • Sample 2: DNA from colony 2
    • Incubated at 37°C for 15 min
  • Electrophoresis
    • 1kbt ladder - 10μL
    • Sample 1 KAH126/MV2 - 15μL
    • Sample 2 KAH126/MV2- 15μL
    • Gel ran for 45 min
Today's gel
15 μL/lane; 1% agarose; Ladder
  • Two Bands in each sample
    • First bands approx 4500bp
    • Second bands approx 2000bp



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