Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/07/14

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07/14/2016

Ran a TypeIIs Cloning Protocol with pBta3 and Bta3R_MRV

Reagent Volume (uL)
Vector (100ng)0.2
Insert (1:250 dilution)2
10x FD2
DTT1
ATP 1
BbsI1
Ligase (T4)0.5
Water 12.3
Total20


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