Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/07/07

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07/07/2016

  • Look at transformation plates from yesterday!
  • 5 plates: CerR, CerR_vector_control, LasR, LasR_vector_control, and negative (water only) control.
  • Only LasR had colonies! 3 colonies on LasR plate.
  • Make liquid cultures for the 3 LasR colonies. Crossing fingers and hoping this is it!!
  • CerR: try long transformation? Or verify promoter product on gel


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