Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/21

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07/21/2015

Transformed:

  • pEsa_MRV + EsaR
  • LasR_MRV + pLas
  • pEsa_MRV, digested with E+X; backbone only control
  • pEsa_MRV, digested with E+X backbone only and treated with ligase
  • LasR_MRV digested with BbsI; backbone only control
  • LasR_MRV, digested with BbsI backbone only and treated with ligase

Ligase and no-ligase controls were created to help troubleshoot.The last LasR_MRV + pLas ligation was not successful (only one colony on plate, and 3 colonies on backbone plate). By doing two controls I hope to trace the problem: is the backbone ligating back to itself, or was it not fully digested?



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